Coding
Part:BBa_K1550007:Design
Designed by: Joel Tyson Group: iGEM14_BUGSS_Baltimore (2014-10-17)
Pfu DNA Polymerase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1209
Illegal XbaI site found at 1919 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1209
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1209
Illegal BglII site found at 764
Illegal BamHI site found at 839 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1209
Illegal XbaI site found at 1919 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1209
Illegal XbaI site found at 1919 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1866
Illegal SapI site found at 354
Illegal SapI.rc site found at 633
Design Notes
The main considerations were enzyme extraction and purification. We plan to test specific enzyme extraction and purification protocols and characterize Pfu polymerase efficiency in PCR.
Source
We attempted to clone contaminated DNA in commercial enzyme stocks.