Coding

Part:BBa_K1550007:Design

Designed by: Joel Tyson   Group: iGEM14_BUGSS_Baltimore   (2014-10-17)


Pfu DNA Polymerase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1209
    Illegal XbaI site found at 1919
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1209
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1209
    Illegal BglII site found at 764
    Illegal BamHI site found at 839
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1209
    Illegal XbaI site found at 1919
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1209
    Illegal XbaI site found at 1919
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1866
    Illegal SapI site found at 354
    Illegal SapI.rc site found at 633


Design Notes

The main considerations were enzyme extraction and purification. We plan to test specific enzyme extraction and purification protocols and characterize Pfu polymerase efficiency in PCR.


Source

We attempted to clone contaminated DNA in commercial enzyme stocks.

References